Abstract:

Evaluating the impact of organic matter and sample processing techniques on RNA detection using environmental samples

Grace E. Houston, DVM; Allison K. Blomme, BS; Olivia L. Harrison, MS; Jianfa Bai, PhD; Jason C. Woodworth, PhD; Cassandra K. Jones, PhD; Elizabeth G. Poulsen-Porter, MS; Chad B. Paulk, PhD; Jordan T. Gebhardt, DVM, PhD

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This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.

Keywords: environmental samples, feed safety

Cite as: Houston GE, Blomme AK, Harrison OL, Bai J, Woodworth JC, Jones CK, Poulson-Porter EG, Paulk CB, Gebhardt JT. Evaluating the impact of organic matter and sample processing techniques on RNA detection using environmental samples. J Swine Health Prod 2023;31(2):87-91.

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