Abstract:

Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids

Ronaldo Magtoto, PhD; Betsy Armenta-Leyva, DVM; Precy Dizon-Magtoto, DVM, MS; Ting-Yu Cheng, DVM, PhD; Maria J. Clavijo, DVM, PhD; Clayton Johnson, DVM; Will Lopez, DVM, PhD; David Baum, DVM, PhD; Jeffrey Zimmerman, DVM, PhD; Luis G. Giménez-Lirola, PhD

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The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds

Keywords: Mycoplasma hyopneumoniae, processing fluid, enzyme-linked immunosorbent assay, diagnostic performance

Cite as: Magtoto R, Armenta-Leyva B, Dizon-Magtoto P, Cheng T-Y, Clavijo MJ, Johnson C, Lopez W, Baum D, Zimmerman J, Giménez-Lirola LG. Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids. J Swine Health Prod 2022;30(3):165-170.

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