Abstract:

Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus

Karen M. Harmon, PhD; Sarah A. Abate, BS; Amy J. Chriswell, BS; Wayne A. Chittick, BS; Erin L. Strait, DVM, PhD

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Specimens were tested for porcine reproductive and respiratory syndrome virus by real-time reverse transcriptase polymerase chain reaction (RT-PCR) with two commercial agent-specific assays. Result discrepancy between the two tests was confirmed by conventional RT-PCR, sequencing, or both on six of 423 clinical cases. Neither assay detected every positive case.

Keywords: PCR, PRRSV

RIS citationCite as: Harmon KM, Abate SA, Chriswell AJ, et al. Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus. J Swine Health Prod 2012;20(4):184-188.

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