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SHIC, Collaborating with CFIA, Funds Development of PCR to Detect and Differentiate Chinese PRV

Availability of a highly sensitive and specific polymerase chain reaction (PCR)-based diagnostic assay for rapid differential detection of pseudorabies virus (PRV) variants, such as those now endemic in China, is critical to prevent huge economic losses to the US and Canadian pork industries if these strains enter North America and cause an outbreak. A single-tube triplex real-time-PCR assay for differential detection of variant strains of PRV has been developed and evaluated in a project funded by the Swine Health Information Center (SHIC) and Canadian Food Inspection Agency (CFIA). The triplex real-time PCR assay developed in this project could be used as a rapid diagnostic tool for foreign animal disease detection in North America or for surveillance and in epidemiological studies in countries, like China, where both classical and variant strains are endemic. The assay is also able to differentiate wild-type PRV from the gE-deletion PRV mutant marker vaccines.

SHIC Issues a Call for Proposals for Improved Oral Fluids PCR Sensitivity

Recent unpublished, observational research indicates oral fluids (OF), when collected by rope as an aggregate sample, could be a good sample for rapid detection of African swine fever virus (ASFv). However this experimental data also suggests that there is potential for false negative testing results. Therefore, the Swine Health Information Center (SHIC) is calling for proposals to develop methods to improve the detection of low levels of nucleic acid in OF through enhancements to sample pre-extraction treatment(s) or through improved extraction methodologies compatible with the high throughput testing currently done in NAHLN laboratories.

SHIC Sponsored Research in Vietnam Looks at Risk of ASF Transmission via Boar Studs

The common practice of artificial insemination (AI) in pork production often involves outside equipment, specialized crews, and off-site boar studs. Because AI has been linked to the spread of several pig disease outbreaks, the Swine Health Information Center (SHIC) is using USDA-FAS grant funds to sponsor research in Vietnam on the risk of introducing African swine fever (ASF) via this route. Specifically, the study will examine potential introduction of ASF to a sow farm as a result of semen movement from apparently healthy boar studs located in an ASF disease control area. Conducted by a team from the University of Minnesota with academic partners from the Vietnam National University of Agriculture, the study will include a pro-active risk assessment (RA) systematically evaluating the potential risk of semen movements during an outbreak. Resulting information will help the US swine industry continue to prepare for foreign animal disease issues as well as implement science-based prevention protocols.

Global Action Needed Now to Halt Spread of Deadly Pig Disease

The escalation of the spread of African swine fever (ASF) has placed most of the world’s domestic and wild pig populations under direct threat. To support countries’ efforts to protect economies and food security, the World Organisation for Animal Health (OIE) and the Food and Agriculture Organization of the United Nations (FAO) today launch a joint initiative for the Global Control of ASF. [Source: OIE 20 July 2020]