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SHIC Pursues Diagnostic Test Capable of Detecting Multiple Swine Viruses Simultaneously from Field Samples

Researchers Drs. Noelle Noyes, Montserrat Torremorell, and colleagues at the University of Minnesota developed a workflow called TELSVirus, or Target-Enriched Long-Read Sequencing of Virus, that enables the real-time detection and genomic characterization of multiple viral pathogens from a single sample in a relatively short turnaround time (approximately 24 hours). As part of a SHIC-funded study, the researchers’ main objective was to apply the TELSVirus workflow to porcine oral fluid samples to detect and characterize genomes of target viral pathogens.

SHIC Helps Refine PCV3 Case Definition with VDL Study

Currently, diagnosis of porcine circovirus type 3 is based on quantifying viral DNA by PCR and occasional confirmation by in situ hybridization of lesions associated with PCV3 infection. However, PCV3 has been detected in clinically and subclinically infected animals, and data to help standardize the PCV3 case definition is needed. A SHIC-funded study conducted by principal investigator Dr. Pablo Pineyro, Iowa State University, provided valuable insights into the diagnosis and prevalence of PCV3 in reproductive failure and surveillance cases submitted to the ISU-VDL as well as the synergism of PCV3 with PRRSV and PCV2.

SHIC Funds Regional Swine Disease Warning Tool Development and Testing

With funding provided by SHIC, the Morrison Swine Health Monitoring Project team at the University of Minnesota developed and tested a tool to enable timely communication of regional disease activity, The Early Regional Occurrence Warning project. TEROW represents additional infrastructure to respond to emerging diseases and highlights efforts towards preparedness, as it can be utilized for a wide range of diseases with the application of standardized monitoring through MSHMP.

SHIC Wean-to-Harvest Biosecurity: Investigating Manure Pumping Effects on Disease Interim Report

With funding from SHIC, the Foundation for Food & Agriculture Research, and Pork Checkoff, a team comprised of Dr. Daniel C. L. Linhares, Dr. Gustavo de Sousa e Silva, Dr. Ana Paula Poeta Silva, and Daniel Moraes with the Iowa State University College of Veterinary Medicine have begun a study examining manure pumping effects on disease onset in wean-to-finish pigs. Their objective is to identify practices related to manure pumping that can be managed to decrease incidence of disease onset in these pig populations. Their study will identify risk factors associated with disease onset in wean-to-finish sites following manure pumping and spread as well as detection of PRRSV and PEDV in pigs and environmental surfaces of wean-to-finish sites before and after manure pumping. The interim report reveals learnings to date.

Porcine Sapovirus Webinar Demonstrates Need for Diligence in Diagnostics

The Swine Health Information Center and American Association of Swine Veterinarians hosted a webinar on porcine sapovirus, a potentially emerging disease, on August 30 with 394 registrants from 22 countries. Speakers shared diagnostic and field experience with PSaV including clinical presentation, production impacts, control/response strategies, and other insights. Cumulatively, presenters offered advice on the risks of narrowly focusing on a single pathogen during a diagnostic investigation, which may result in missed detections of emerging or less common pathogens. A definitive lab diagnosis is necessary to know which pathogen or combination of pathogens are the cause of clinical disease in your swine herd.

SHIC Issues RFP for Tongue Tip Diagnostic Sample Research

Research proposals investigating tongue tips as a novel diagnostic sample type are now being accepted as part of a recently released SHIC RFP. Diagnostic investigation of tongue tips from stillborn pigs and neonatal mortalities may be used to gain information about PRRSV, or other pathogens, circulating during gestation and being vertically transmitted from the gestating sow to her litter. Tongue tips might also be used to monitor viral pathogen horizontal transmission in a variety of ages of pigs. Research into the use of tongue tip monitoring will help provide producers and their veterinarians with critical information that they may need to effectively respond to an emerging disease outbreak.