TY - JOUR AU - Knittel, JP AU - Larson, DI AU - Harris, DL TI - United States isolates of Lawsonia intracellularis from porcine proliferative enteropathy resemble European isolates T2 - Journal of Swine Health and Production JF - Journal of Swine Health and Production J2 - JSHAP SN - 1537-209X DP - American Association of Swine Veterinarians PB - American Association of Swine Veterinarians DA - 1996/May// PY - 1996 VL - 4 M1 - 3 IS - 3 M2 - 118 SP - 118-122 UR - https://www.aasv.org/shap/abstracts/abstract.php?v4n3p118 KW - swine KW - Lawsonia intracellularis KW - isolate N2 - Objective--To compare isolates of Lawsonia intracellularis from United States pigs to European isolates. Design and procedure--Three isolates of L. intracellulariswere isolated from the intestines of United States pigs with porcine proliferative enteropathy. The organisms were grown and passaged in a rat enterocyte cell line (IEC-18). Growth was not associated with morphological cell change and was monitored by immunostaining of the cells using a monoclonal antibody and by polymerase chain reaction (PCR). The PCR products were evaluated by DNA sequencing and compared to a known sequence from L. intracellularis.Results- Lawsonia intracellularis is a microaerophilic, Gram-negative, curved or S-shaped bacillus and is an obligately intracellular organism. These isolates resemble European isolates by PCR, monoclonal antibody reactions, and growth requirements. ER -