TY - JOUR AU - Prickett, JR AU - Cutler, S AU - Kinyon, JM TI - Stability of porcine reproductive and respiratory syndrome virus and antibody in swine oral fluid T2 - Journal of Swine Health and Production JF - Journal of Swine Health and Production J2 - JSHAP SN - 1537-209X DP - American Association of Swine Veterinarians PB - American Association of Swine Veterinarians DA - 2010/Jul// PY - 2010 VL - 18 M1 - 4 IS - 4 M2 - 187 SP - 187-195 L2 - https://www.aasv.org/shap/issues/v18n4/v18n4p187.html UR - https://www.aasv.org/shap/abstracts/abstract.php?v18n4p187 L1 - https://www.aasv.org/shap/issues/v18n4/v18n4p187.pdf KW - swine KW - antibody KW - oral fluid KW - nucleic acid KW - porcine reproductive and respiratory syndrome virus KW - PRRS KW - PRRSV N2 - Objective: To evaluate the stability of porcine reproductive and respiratory syndrome virus (PRRSV) and anti-PRRSV antibodies in oral fluid as a function of time and temperature.Materials and methods: A 4-L pool of swine oral fluid was collected from 16-week-old finisher pigs. To ensure uniform, quantifiable levels of virus and antibody over time, 4 mL of PRRSV isolate ISU-P containing 1 × 1012 RNA copies per mL and 10 mL of concentrated hyperimmune anti-PRRSV antibodies were added to the pool. The pool was then divided into three equal portions: no treatment, chlorhexidine digluconate at 0.01% by volume, and isothiazolinone at 3 ppm. Each treatment was tested in triplicate at each of five temperatures (-20°C, 4°C, 10°C, 20°C, and 30°C). Samples were removed at specific intervals (0, 12, 24, 48, 72, 144, 216, and 288 hours), stored at -80°C, and then assayed for PRRSV RNA; IgM, IgA, and IgG PRRSV-specific antibody; and culturable bacteria per mL.Results: The stabilities of anti-PRRSV antibody and detectable PRRSV were temperature-dependent, with antimicrobial treatment providing no improvements in stability at lower temperatures. Both virus and antibody were stable at ≤ 10°C over 12 days of storage.Implication: Conventional serum storage protocols (freezing or refrigeration at 4°C) preserves PRRSV and anti-PRRSV antibody in oral-fluid diagnostic samples without the use of preservatives. ER -